꽃송이버섯 추출물의 항염활성 효과
Anti-inflammatory effects of Sparassis crispa extracts
Abstract
Sparassis crispa is a medicinal mushroom, which has been reported to have anti-cancer effect. In this study,we designed to investigate the effects of Sparassis crispa extracts on the production of nitric oxide (NO) in LPS-stimulatedRAW264.7 cells. The pre-treatment of the extracts prior to add LPS in RAW264.7 cells suppressed NO production and iNOSexpression at protein and mRNA levels. The phosphorylation of IĸBα was inhibited by the extracts, which was induced throughsuppressing the activation of NF-ĸB. Sparassis crispa extracts showed the effect on the down-regulation of STAT-1 activation ina dose-dependent manner. In LPS-stimulated RAW264.7 cells, NF-ĸB was translocated into the nucleus, while the treatmentof Sparassis crispa extracts induced to sequestered NF-ĸB in the cytosol. These experimental results determined that Sparassiscrispa extracts play a inhibitory role in inflammatory reactions via regulating NO production, which suggests potential as acomponent of inflammatory drugs.
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Reference
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20.Xie, Q., Kashiwabara, Y. and Nathan, C. 1994. Role of transcription factor NF-κB/Rel in induction of nitric oxide synthase, J. Biol. Chem. 269: 4705-4708.
21.Yamamoto, K., Kimura, T., Sugitachi, A. and Matsuura, N. 2009. Anti-angiogenic and anti-metastatic effects of. β.-1, 3-Dglucan purified from hanabiratake, Sparassis crispa, Biol. Pharma. Bull, 32: 259-263.
22.Yoon, W. J., Ham, Y. M., Kim, S. S., Yoo, B. S., Moon, J. Y., Baik, J. S., Lee, N. H. and Hyun, C. G. 2009. Suppression of pro-inflammatory cytokines, iNOS, and COX-2 expression by brown algae Sargassum micracanthum in RAW 264.7 macrophages, EurAsia J. BioSci. 3: 130-143.
2.Andrej, J., Shailesh, D., Qing-li, W., James, S. and Daniel, S. 2011. Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling, Nutr. J. 10: 52.
3.Barros, L., Baptista, P., Estevinho, L. M. and Ferreira, I.C.F.R. 2007. Effect of fruiting body maturity stage on chemical composition and antimicrobial activity of Lactarius sp. mushrooms, J. Agr. Food Chem. 55: 8766-8771.
4.Cho, S. Y., Park, S. J., Kwon, M. J., Jeong, T. S.,Bok, S. H., Choi, W. Y., Jeong, W. I., Ryu, S. Y. Do, S. H. and Lee, C.S. 2003. Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-κB pathway in lipopolysaccharide-stimulated macrophage, Mol. Cell. Biochem. 243: 153-160.
5.Cirino, G., Distrutti, E. and Wallace, J. L. Nitric oxide and inflammation, Inflamm. Allergy. (2006) 115-119.
6.Farlik, M., Reutterer, B., Schindler, C., Greten, F., Vogl, C., Müller, M. and Decker, T. 2010. Conventional initiation complex assembly by STAT and NF-κB transcription factors regulates nitric oxide synthase expression, Immunity 33: 25-34.
7.Gomez, P. F., Pillinger, M. H., Attur, M., Marjanovic, N., Dave, M., Park, J., Bingham III, C. O., Al-Mussawir, H. and Abramson, S. B. 2005. Resolution of inflammation: prostaglandin E2 dissociates nuclear trafficking of individual NF-κB subunits (p65, p50) in stimulated rheumatoid synovial fibroblasts, J. Immunol. 175: 6924-6930.
8.Guillamón, E., García-Lafuente, A., Lozano, M., D'Arrigo, M., Rostagno, M. A., Villares, A. and Martínez, J. A. 2010. Edible mushrooms: role in the prevention of cardiovascular diseases, Fitoterapia 81: 715-723.
9.Kim, G. Y., Han, M. G., Song, Y. S., Shin, B. C., Shin, Y. I., Lee, H. J., Moon, D. O., Lee, C. M., Kwak, J. Y., Bae, Y. S., Lee, J. D. and Park, Y. M. 2004. Proteoglycan Isolated from Phellinus linteus induces toll-like receptors 2- and 4-mediated maturation of murine dendritic cells via activation of ERK, p38, and NF-κB, Biol. Pharm. Bull. 27: 1656-1662.
10.Kim, H. G., Yoon, D. H., Lee, W. H., Han, S. K., Shrestha, B., Kim, C. H., Lim, M. H., Chang, W., Lim, S. and Choi, S. 2007. Phellinus linteus inhibits inflammatory mediators by suppressing redox-based NF-κB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophage, J. Ethnopharmacol. 114: 307-315
11.Kim, H. S., Kim, J. Y., Ryu, H. S., Park, H. G., Kim, Y. O., Kang, J. S., Kim, H. M., Hong, J. T., Kim, Y. and Han, S. B. 2010. Induction of dendritic cell maturation by β-glucan isolated from Sparassis crispa, Int. Immunopharmacol. 10: 1284-1294.
12.Kim, M. Y., Seguin, P., Ahn, J. K., Kim, J. J., Chun, S. C., Kim, E. H., Seo, S. H., Kang, E. Y., Kim, S. L. and Park, Y. J. 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea, J. Agr. Food Chem. 56: 7265-7270.
13.Kwon, A., Qiu, Z., Hashimoto, M., Yamamoto, K. and Kimura, T. 2009. Effects of medicinal mushroom (Sparassis crispa) on wound healing in streptozotocin-induced diabetic rats, Am. J. Surg. 197: 503-509.
14.Laskin, D. L. and Pendino, K. J. 1995. Macrophages and inflammatory mediators in tissue injury, Annu. Rev. Pharmacol. 35: 655-677.
15.Liang,Y. C., Huang, Y. T., Tsai, S. H., Lin-Shiau, S. Y., Chen, C. F. and Lin, J. K. 1999. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages, Carcinogenesis 20: 1945-1952
16.Moradali, M. F., Mostafavi, H., Ghods, S. and Hedjaroude, G. A. 2007. Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi), Int. Immunopharmacol. 7: 701-724.
17.Raja, B. 2011. Veratric acid, a phenolic acid attenuates blood pressure and oxidative stress in L-NAME induced hypertensive rats, Eur. J. Pharmacol. 671: 87-94.
18.Stefano, D. D., Maiuri, M.C., Iovine, B., Ialenti, A., Bevilacqua, M. A. and Carnuccio, R. 2006. The role of NF-κB, IRF-1, and STAT-1α transcription factors in the iNOS gene induction by gliadin and IFN-γ in RAW 264.7 macrophages, J. Mol. Med. 84: 65-74.
19.Walley, K. R., McDONALD, T. E., Higashimoto, Y. and Hayashi, S. 1999. Modulation of proinflammatory cytokines by nitricoxide in murine acute lung injury, Am. J. Resp. Crit. Care. 160: 698-704.
20.Xie, Q., Kashiwabara, Y. and Nathan, C. 1994. Role of transcription factor NF-κB/Rel in induction of nitric oxide synthase, J. Biol. Chem. 269: 4705-4708.
21.Yamamoto, K., Kimura, T., Sugitachi, A. and Matsuura, N. 2009. Anti-angiogenic and anti-metastatic effects of. β.-1, 3-Dglucan purified from hanabiratake, Sparassis crispa, Biol. Pharma. Bull, 32: 259-263.
22.Yoon, W. J., Ham, Y. M., Kim, S. S., Yoo, B. S., Moon, J. Y., Baik, J. S., Lee, N. H. and Hyun, C. G. 2009. Suppression of pro-inflammatory cytokines, iNOS, and COX-2 expression by brown algae Sargassum micracanthum in RAW 264.7 macrophages, EurAsia J. BioSci. 3: 130-143.