차가버섯 메탄올 추출물의 항산화 및 미백효과
Antioxidant and skin whitening effects of Inonotus obliquus methanol extract
(Received June 11, 2013. Accepted June 20, 2013)
Abstract
This study was initiated to investigate the skin whitening activities of methanol extracts from fruitingbodies of I. obliquus. The total polyphenols and flavonoids contents of I. obliquus methanol extracts were 31.85 mg/g and 28.33 mg/g, respectively. The methanol extract of the mushroom treated on B16/F10 melanoma and NIH3T3cell lines did not show cytotoxic activity. 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity andchelating activity on ferrous ions of I. obliquus methanol extract were lower than those of positive control, tocopheroland BHT. The tyrosinase and L-DOPA inhibitory activities of the extract were lower than those of positivecontrol, kojic acid and ascorbic acid. The tyrosinase and melanin synthesis inhibitory activities of the melanomacells treated with the extract were comparable with positive control, arbutin. The experimental results suggestedthat methanol extract of I. obliquus contained inhibitory activities of tyrosinase and melanin synthesis in the B16/F10 melanoma cells by dose dependent manner. High ultra-violet absorption spectra in the range of 280-350 nmshowed that I. obliquus extract could protect skin from UV radiation damage. Therefore, fruiting bodies of I. obliquuscan be used for developing skin whitening, anti-UV and skin care agents.
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Reference
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2.Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature. 181 : 1199-1200.
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4.Ishihara, Y., Oka, M., Tsunakawa, M., Tomita, K., Hatori, M., Yamamoto, H., Kamei, H., Miyaki, T., Konishi, M., Oki, T. 1991. Melanostatin, a new melanin synthesis inhibitor. Production, isolation, chemical properties, structure and biological activity. J. Antibiotics. 44 : 25.
5.Kim, J. O., Jung, M. J., Choi, H. J., Lee, J. T., Lim. A. K., Hong, J. H. and Kim, D. I. 2008. Antioxidative and biological activity of hot water and ethanol extracts from Phellinus linteus. J. Kor. Soc. Food Sci. Nutr. 37 : 684-690.
6.Kim, M. J. and Ryu, M. J. 2012. Inhibition of melanogenesis and anti-UV properties of Reynoutria elliptica. K. J. Aesthet. Cosmotol. 10 : 961-968.
7.Maeda, K. and M. Fukuda. 1996. Arbutin: mechanism of its depigmenting action in human melanocyte culture. J. Pharmacol. Exp. Ther. 276 : 765-769.
8.Masuda, T., Yamashita, D., Takeda, Y. and Yonemori, S. 2005. Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Biosci. Biotechnol. Biochem. 69 : 197-201.
9.Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radicalscavenging activity of propolis from several region of Argentina. J. Enthnopharm. 71 : 109-114.
10.Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol. Meth. 65 : 55.
11.Nam, B. H., Jo, W. S., Choi, Y. J., Lee, J. Y., Kang, E. Y., Jeong, M. H. and Lee J. D. 2010. Inhibitory effects of melanin secretion on B16 melanoma cell of Cordyceps militaris water extract. Kor. J. Mycol. 38 : 167-171.
12.Pawwelk, J. 1978. Melanoma cells in culture. Methods in Enzymol. 58 : 564-568.
13.Pomerantz, S. H. 1963. Separation, purification and properties of two tyrosinases from hamster melanoma. J. Biol. Chem. 238 : 2351-2354.
14.Rocha-Guzmn, N. E., Gonzlez-Laredo, J. A., Conzalez-Laredo, R. F., Reynoso-Camacho, R., Ramos-Gmez, M., Garcia-Gasca, T., Rodrguez-Muoz, M. E., Guzmn-Maldonado, S. H., Medina-Torres, L. and Lujan-Garca, B. A. 2009. Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves. Food Chem. 115 : 1320-1325.
15.Shashkina, M. Y., Shashkin, P. N. and Sergeev, A. V. 2006. Chemical and medicobiological properties of Chaga (review). Pharm. Chem. J. 40(10) : 37-44.
16.Shim, S. M., Im, K. H., Kim, J. W., Shim, M. J., Lee, M. W. and Lee, T. S. 2003. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31 : 155-160.
17.Swain, T., Hillis, W. E. and Ortga, M. 1959. Phenolic constituents of Prunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10 : 83-88.
18.Xu, X. M., Jun, J. Y. and Jeong, I. H. 2007. A study on the antioxidant activity of Hae-Songi mushroom (Hypsizigus mamoreus) hot water extract. J. Kor. Soc. Food Sci. Nutr. 36 : 1351-1357.
19.Yena, G. C., Duhb, P. D. and Tsaia, L. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem. 79 : 307-313.
20.Yoon, K. N., Alam, N., Lee, K. R., Shin, P. G., Cheong, J. C., Yoo, Y. B. and Lee, T. S. 2011. Antioxidant and antityrosinase activities of various extracts from the fruiting bodies of Lentinus lepideus. Molecules. 16 : 2334-2347.