PCR 다형성 분석에 의한 비늘버섯 속 계통의 유연관계 분석
Phylogenetic relationships in different strains of Pholiota species based on PCR polymorphism
(Received June 11, 2013. Accepted June 20, 2013)
Abstract
Pholiota species were collected from different geographical regions of the world. Genetic diversityand phylogenetic relationships were analyzed by rDNA-ITS sequences and RAPD polymorphism. The sizes ofrDNA-ITS PCR amplicons of Pholiota spp. varied from 233~271, 158~223 and 174~219 bp, respectively. A phylogenetictree was constructed on the ITS region sequences and Pholiota strains were classified into 8 clusters.Twenty strains in seven Pholiota spp. were classified into seven clusters by RAPD polymorphism using 15 arbitraryprimers. Our experimental results suggested that rDN-ITS and RAPD analysis are useful tool for classifying Pholiotaspp. and strains.
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2.Alam, N., Shim, M. J., Lee, M. W., Shin, P. G., Yoo, Y. B. and Lee, T. S. 2009b. Vegetative growth and phylogenetic relationship of commercially cultuvated strains of Pleurotus eryngii based on ITS sequence and RAPD. Mycobiology 37 : 258-266.
3.Caetano, A. G. and Gresshoff, P. M. 1997. DNA markers: Protocols Applications, and Overviews. Wiley-Vch, N.Y. pp. 364.
4.Cubero, O. F., A. Crespo, J. Fatehi and P. D. Bridge. 1999. DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst. Evol. 216 : 243-249.
5.Felsenstein, J., 1985. Confidence limits on phylogenies: and approach using the bootstrap. Evol. 39 : 789-791
6.Khan, P. and Garcha, H. S. 1984. Pleurotus mushroom, a source of food protein. Mush. Newslett. Trop. 4 : 9-14
7.Lim, Y. J., Lee, C. Y., Park, J. E., Kim, S. W. Lee, H. S. and Ro, H. S. 2010. Molecular genetic classification of Hypsizigus marmoreus and development of strain-specific DNA markers. Kor. J. Mycology 38(1) : 34-39.
8.Nei, M. and Li, W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. 76 : 5269-5273.
9.Park, H. G., Ko, H. G., Kim, S. H. and Park W. M. 2004. Molecular identification of Asian isolares of medicinal mushroom Hericium erinaceum by phylogenetic analysis of nuclear ITS rDNA. J. Microbiol. Technol. 14 : 816-821.
10.Park, W. H. and Lee, H. D. 2003. Illustrated book of Korean medicinal mushrooms. Kyo-Hak Publishing Co. Ltd. Seoul.
11.Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstruction phylogenetic trees. Mol. Biol. Evol. 4 : 406-425.
12.White, T. J., Burns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Eds., M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White. Academic Press, San Diego, California, 482 p.
13.Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18 : 6531-6535.