천마버섯(Armillaria gallica) 자실체 조직배양체의 특성
Characterization of cultures isolated from fruiting body tissue in Armillaria gallica
(Received May 28, 2013. Accepted June 17, 2013)
Abstract
The fruiting body of honey mushroom, Armillaria gallica, was collected from Gastrodia elata cultivatedfields. Pure cultures were isolated from fruiting body tissue of the mushrooms, and cultured on MCM(mushroom complete medium) or PDA (potato dextrose agar) medium. Then, 12 different types of mycelial growthcharacteristics such as growth rate, colony morphology and rhizomorph formation were obtained. The vitality ofthe mycelial growth and rhizomorph formation of the fruiting body culture isolates were better on MCM thanPDA, suggesting that the optimal culture medium for A. gallica mycelia was MCM. To observe the feature of colonymorphology, the subculture of isolates were incubated on MCM. Consequently, we could find the segregated ordifferentiated colony morphology from isolate type 11 that was similar morphology to isolate type 12. For phylogeneticanalysis of the 12 isolates, RAPD (Random Amplified Polymorphic DNA) were performed. The isolatetype 12 was not only shown different band patterns of RAPD variation in other 11 isolates, but also commercialstrain known as Chunmagyun No. 1. Among the tissue culture isolates of fruiting, strains with better mycelialgrowth characteristics than Chunmagyun No. 1 were selected. We expect that the new strain can be substitutedto commercial strain Chunmagyun No. 1.
Armillaria gallica,Commercial strain Chunmagyun No. 1. Fruiting body culture isolates,Honey mushroom,RAPD,Rhizomorph formation
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Reference
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2.성재모, 양근주, 김수호, T. Harrinton. 1997. 생물학적 특성과 DNA분석을 이용한 한국내 Armillaria 속균의 분류. 한국균학회지 25 : 46-67.
3.성재모, 정범식, 양근주, 이현경, T. Harrinton. 1995. Armillaria 속균을 이용한 천마의 생산. 한국균학회지 23 : 61-70.
4.오진아, 이찬중, 정종천, 유영복. 2012. rDNA의 ITS 부위 염기서열 분석에 의한 Armillaria속 수집 균주의 유전적인 유연관계 분석. 한국버섯학회지 10(3) : 143-149.
5.유영복, 오진아, 오연이, 문지원, 신평균, 장갑열, 공원식. 2013. 천마버섯(Armillaria gallica)의 생태학적 서식지 조사. 한국버섯학회지 11(1) : 36-40.
6.Carvalho, D. B., Smith, M. L. and Anderson, J. B. 1995. Genetic exchange between diploid and haploid mycelia of Armillaria gallica. Mycol. Res. 99(6) : 641-647.
7.Kevei, F. and Peberdy, J. F. 1979. Induced segregation in interspecific hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion. Mol. Gen. Genet. 170 : 213-218.
8.Raper C. A, raper J. R, and Miller R. E. 1972. Genetic analysis of the life cycle of Agaricus bisporus. Mycologia. 64 : 1088-1117.
9.Sung, J. M., Cha, J. Y. and Kim, Y. S. 1989. Identification and culture of A. mellea complex associated with root rot disease of Korean pine. Korean J. Plant Pathol. 5(3) : 317-327.
10.Sung, J. M., Yang, K. J., Lee, H. K. and Harrigton, T. C. 1994. Studies on Korean species of Armillaria. Kor. J. Pl. Path. 10(4) : 261-269.
11.Yoo, Y. B. and Shin, P. G. 1996. Intergeneric transfer of isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida. Korean J. Mycology 24(2) : 111-126.