영지버섯 균주별 자실체의 베타글루칸과 폴리페놀 함량 비교
Comparative analysis of useful β-glucan and polyphenol in the fruiting bodies of Ganoderma spp.
(Received September 10, 2013 / Revised September 25, 2013 / Accepted September 27, 2013)
Abstract
This study was carried out to compare the medicinal effects of various fruiting body of Ganodermaspecies and Cordyceps militaris, Phelinus linteus extracts. β-glucan and polyphenol are useful ingredientin mushrooms and they were known to have antioxidant activity. We analyzed β-glucan and polyphenol contentsof fruiting body of Ganoderma spp., Cordyceps militaris, and Phellinus linteus. Most Ganoderma spp.exhibited β-glucan contents of 15 to 20%. Cordyceps militalis showed the highest β-glucan level of 25%. Interestingly,eight strains of Ganoderma spp. was analyzed to have higher contents of β-glucan than Phelinus linteus.Polyphenol contents was measured after extraction with different solvents. (D.W., 70% EtOH, 80%MeOH) The level of polyphenol in ASI 7020 strain was at maximum in the water extraction and ASI 7086showed the highest level in the 70% EtOH extraction. The amounts of polyphenol in strain ASI 7113 wasat maximum in the 80% MeOH extraction.
- 0096-01-0011-0003-9.pdf1.82MB
Reference
1.Kim SS, Kim YS. 1990. Korean mushrooms. Yupoong Publishing Co., Seoul, Korea. p 3.3. Kim HJ, Lee IS. 2004. Anti-mutagenic and cytotoxic effects of Korean wild mushrooms extracts. Korean J Food Sci Technol 36 : 662-668.
2.Choi SJ, Lee YS, Kim JK, Lim SS. 2010. Physiological activities of extract from edible mushrooms. J Korean Soc Food Sci Nutr 39 : 1087-1096.
3.Yang JH, Lin HC, Mau JL. 2002. Antioxidant properties of several commercial mushrooms. Food Chem 77 : 229-235.
4.Mus JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35 : 519-526.
5.Choi YH, Kim MJ, Lee HS, Yun BS, Hu C, Kwak SS. 1998. Antioxidative compounds in aerial parts of Potentilla fragariodes. Korean J Pharmacogn 29 : 79-85.
6.A. O. A. C. 1984. Official methods of analysis, 14th ed., Association of Official Analytical Chemists. Washington, D. C. Agnese, A. M., C. Perez, and J. L. Cabrera. 2001. Adesmia aegiceras : antimicrobial activity and chemical study. Phytomedicine. 8(5) : 389-394. 
7.Blosi, M. S. 1958. Antioxidant determinations by use of a stable free radical. Nature. 26 : 1199-1200.
8.Chan, G.C., W.K. Chan, and D.M. Sze, The effects of betaglucan on human immune and cancer cells. J. Hematol. Oncol. 2 : 25 (2009).
9.Choi, J. S., S. H. Park, and I. S. Kim. 1989. Studies on the active principles of wild vegetables on biotransformation of drug. Kor. J. Pharmacogn. 20 : 117-122.
10.Chon, S. U., C. H. Bae, and S. C. Lee. 2012. Antioxidant and cytotoxic potentials of methanol extracts from Taraxacum officinale F. H. Wigg. at different plant parts. Kor. J. Plant Res. 25(2) : 232-239.
11.Dellinger, E.P., et al., Effect of PGG-glucan on the rate of serious postoperative infection or death observed after high risk gastrointestinal operations. Betafectin Gastrointestinal Study Group. Arch. Surg. 134(9) : 977-983 (1999).
12.Liu, J., et al., Combined yeast-derived beta-glucan with antitumor monoclonal antibody for cancer immunotherapy. Exp.Mol. Pathol. 86(3) : 208-214 (2009).
13.Mueller, A., et al., The influence of glucan polymer structure and solution conformation on binding to (1-->3)-beta-D-glu-can receptors in a human monocyte-like cell line. Glycobiology. 10(4) : 339-346 (2000).
14.Novak, M. and V. Vetvicka, Beta-glucans, history, and the present: immunomodulatory aspects and mechanisms of action. J Immunotoxicol. 5(1) : 47-57 (2008)
15.Ramberg, J.E., E.D. Nelson, and R.A. Sinnott, Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr. J. 9 : 54 (2010).
16.Schepetkin, I.A. and M.T. Quinn, Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. Immunopharmacol. 6(3) : 317-333 (2006).
17.Wakshull, E., et al., PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: evidence for a glycosphingolipid beta-(1,3)-glucan receptor. Immunopharmacology. 41(2) : 89-107 (1999).
2.Choi SJ, Lee YS, Kim JK, Lim SS. 2010. Physiological activities of extract from edible mushrooms. J Korean Soc Food Sci Nutr 39 : 1087-1096.
3.Yang JH, Lin HC, Mau JL. 2002. Antioxidant properties of several commercial mushrooms. Food Chem 77 : 229-235.
4.Mus JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35 : 519-526.
5.Choi YH, Kim MJ, Lee HS, Yun BS, Hu C, Kwak SS. 1998. Antioxidative compounds in aerial parts of Potentilla fragariodes. Korean J Pharmacogn 29 : 79-85.
6.A. O. A. C. 1984. Official methods of analysis, 14th ed., Association of Official Analytical Chemists. Washington, D. C. Agnese, A. M., C. Perez, and J. L. Cabrera. 2001. Adesmia aegiceras : antimicrobial activity and chemical study. Phytomedicine. 8(5) : 389-394.
7.Blosi, M. S. 1958. Antioxidant determinations by use of a stable free radical. Nature. 26 : 1199-1200.
8.Chan, G.C., W.K. Chan, and D.M. Sze, The effects of betaglucan on human immune and cancer cells. J. Hematol. Oncol. 2 : 25 (2009).
9.Choi, J. S., S. H. Park, and I. S. Kim. 1989. Studies on the active principles of wild vegetables on biotransformation of drug. Kor. J. Pharmacogn. 20 : 117-122.
10.Chon, S. U., C. H. Bae, and S. C. Lee. 2012. Antioxidant and cytotoxic potentials of methanol extracts from Taraxacum officinale F. H. Wigg. at different plant parts. Kor. J. Plant Res. 25(2) : 232-239.
11.Dellinger, E.P., et al., Effect of PGG-glucan on the rate of serious postoperative infection or death observed after high risk gastrointestinal operations. Betafectin Gastrointestinal Study Group. Arch. Surg. 134(9) : 977-983 (1999).
12.Liu, J., et al., Combined yeast-derived beta-glucan with antitumor monoclonal antibody for cancer immunotherapy. Exp.Mol. Pathol. 86(3) : 208-214 (2009).
13.Mueller, A., et al., The influence of glucan polymer structure and solution conformation on binding to (1-->3)-beta-D-glu-can receptors in a human monocyte-like cell line. Glycobiology. 10(4) : 339-346 (2000).
14.Novak, M. and V. Vetvicka, Beta-glucans, history, and the present: immunomodulatory aspects and mechanisms of action. J Immunotoxicol. 5(1) : 47-57 (2008)
15.Ramberg, J.E., E.D. Nelson, and R.A. Sinnott, Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutr. J. 9 : 54 (2010).
16.Schepetkin, I.A. and M.T. Quinn, Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. Immunopharmacol. 6(3) : 317-333 (2006).
17.Wakshull, E., et al., PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: evidence for a glycosphingolipid beta-(1,3)-glucan receptor. Immunopharmacology. 41(2) : 89-107 (1999).