기와층버섯 자실체의 메탄올 및 열수추출물의 항산화, 항염증 및 아세틸콜린에스테라제 저해 활성
Antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of fruiting bodies of Phellinus xeranticus
(Received December 4, 2013 / Revised December 23, 2013 / Accepted December 30, 2013)
Abstract
Phellinus xeranticus is an medicinal mushroom belongs to Family Hymenochaetaceae of Polyporales,Basidiomycota. The purpose of this study was to investigate the antioxidant, anti-inflammatory and anti-acetylcholinesteraseactivities of methanol and hot water extracts prepared from fruiting bodies of Phellinus xeranticus. Besides measuringof 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, a reducing power and a chelating activity onferrous ions were also measured to evaluate the antioxidant activity of the extracts. To measure the anti-inflammatory activitiesof the extracts, nitric oxide(NO) production from lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cellsand carrageenan-induced acute hind paw edema of rats were investigated. The results showed that the extracts have excellentDPPH scavenging and chelating activity on the ferrous ions compared with positive controls. The nitric oxide (NO)production in LPS-induced RAW 264.7 macrophage cells were decreased as the concentration of the mushroom extractsincreased. Significant reduction of paw edema of rats were observed at 2~6 h after treatment of methanol and hot-waterextracts with 50 mg/kg concentration to the rats which are induced acute hind paw edema by carrageenan administration.The anti-acetylcholinesterase activity of the methanol extract of the mushroom showed 83.34% inhibition on AcHE whichis lower than that of positive control galanthamine. The experimental results suggested that methanol and hot-water extractsof Phellinus xeranticus fruiting bodies might be used for good sources of antioxidant, anti-inflammatory and anti-acetylcholinesteraseagents.
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4.Oh, M. H., Houghton, P. J., Whang, W. K. and Cho, J. H. 2004. Screening of Korean herbal medicines used to improve cognitive function for anti-cholinesterase activity. Phytomedicine 11 : 544-548.
5.Behl C. 1999. Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches. Prog. Neurobiol. 57 : 301-323.
6.Park, W. H. and Lee, H. D. 2003. Illustrated book of Korean medicinal mushrooms. Kyo-Hak Publishing Co. Ltd. Seoul.
7.Shim, S. M., Im, K. H., Kim, J. W., Shim, M. J., Lee, M. W. and Lee, T. S. 2003. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31 : 155-160.
8.Swain, T., Hillis, W. E. and Ortga, M. 1959. Phenolic constituents of Prunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10 : 83-88.
9.Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radicalscavenging activity of propolis from several region of Argentina. J. Enthnopharm. 71 : 109-114.
10.Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol. Meth. 65 : 55-63.
11.Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature. 181 : 1199-1200.
12.Gulcin, I., Buyukokuroglu, M. E., Oktay, M. and Kufrevioglu. O. I. 2003. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. Pallsiana (Lamb.) Holmboe. J. Ethnopharmacol. 86 : 51-8
13.Winter, C. A., Risley, E. A. and Nuss, G. W. 1962. Carrageenan induced edema in the hind paw of rat as an assay for anti-inflammatory activity, Proc. Soc. Exp. Biol. Med. 111 : 544-547.
14.Ellman, G.L., Courtney, K. D., Andres, V. Jr. and Featherstone, R. M. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7 : 88-95.
15.Leong, L. P. and Shui, G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76 : 69-75.
16.Jo, S. H., Jin, G. E., Choi, J. S., Yun, H. S., Yu, Y. B. and Park, K. M. 2010. Physiological activity of Flammulina velutipes sp. ethanol extract. J. Mush. Sci. Prod. 4 : 150-156.
17.Um, S. N., Jin, G. E., Park, K. W., Yu, Y. B. and Park, K. M. 2010. Physiological Activity and Nutritional Composition of Pleurotus Species. Korean J. Food Sci. Technol. 42 : 90-96.
18.Lee, Y. L., Yen, M. and Mau, J. L. 2007. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem. 104 : 1-9.
19.Mau, J. L., Chang, C. N., Huang, S. J. and Chen, C. C. 2004. Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta, and Termitomyces albuminosus mycelia. Food Chem. 87 : 111-118.
20.Jang, H. j., Kim, A. K., Pyo, M. Y. and Yang K. S. 2007. Inhibitors of nitric oxide synthase from Phellinus pini in murine macrophages. Yakhak Hoeji. 51 : 430-434.
21.Fangkrathok, N., Junlatat, J. and Sripanidkulchai, B. 2013. In vivo and in vitro anti-inflammatory activity of Lentinus polychrous extract. J. Ethnopharm. 147 : 631-637.
22.Lee, H. C., Hwang, S. G., Lee, Y. C., Kim, D. G. Park, Y. J., Kim, T. E., Kim, M. D. and Jeon, B. H. 2002. Screening of the acetylcholinesterase inhibitors from water extracts of the medicinal plants. Kor. J. Orien. Med. Pathol. 6 : 215-219.