흰망태버섯 자실체의 메탄올 및 열수추출물의 항산화 및 항염증 활성
Antioxidant and anti-inflammatory activities of fruiting bodies of Dyctiophora indusiata
(Received December 3, 2013 / Revised December 23, 2013 / Accepted December 30, 2013)
Abstract
Dictyophora indusiata is an edible mushroom belongs to Family Phallaceae of Phallales, Basidiomycota.The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of methanol and hot waterextracts prepared from fruiting bodies of Dictyophora indusiata. Besides measuring of 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity, a reducing power and a chelating activity on ferrous ions were also measuredto evaluate the antioxidant activity for those extracts. To measure the anti-inflammatory activities for the extracts, nitricoxide(NO) production from lipopolysaccharide(LPS) treated RAW 264.7 macrophage cells and carrageenan-induced acutehind paw edema of rats were investigated. The results showed that the extracts have excellent DPPH scavenging andchelating activity on the ferrous ions compared with positive control. The nitric oxide(NO) production in LPS-stimulatedRAW 264.7 macrophage cells were decreased as we increased the concentration of the mushroom extracts. Significantreduction of paw edema of rats were observed at 2~6 h after treatment of methanol and hot-water extracts with 50 mg/kg concentration to the rats which are induced acute hind paw edema by carrageenan administration. Therefore, the experimentalresults suggested that methanol and hot-water extracts of Dictyophora indusiata fruiting bodies might be used fornatural sources of antioxidant and anti-inflammatory agents.
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2.Ahn, E. K., Jeon H. J., Lim, E. J., Jung, H. J. and Park, E. H. 2007. Anti-inflammatory and anti-angiogenic activities of Gastrodia elata Blume. J. Enthnopharm. 110 : 476-482.
3.Park Y. M., Won, J. H., Kim, Y. H., Choi, J. W., Park, H. J. and Lee, K. T. 2005. In vivo and in vitro antiinflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus. J. Ethnopharm. 101 : 120-128.
4.Gross R. L. and Newberne, P. M. 1980. Role of nutrition in immunologic function. Physiol. Rev. 60 : 188-302.
5.Park, W. H. and Lee, H. D. 1997. Illustrated book of Korean medicinal mushrooms. Kyo-Hak Publishing Co., Ltd. pp. 576. Seoul, Korea.
6.Shim, S. M., Im, K. H., Kim, J. W., Shim, M. J., Lee, M. W. and Lee, T. S. 2003. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31 : 155-160.
7.Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radicalscavenging activity of propolis from several region of Argentina. J. Enthnopharm. 71 : 109-114.
8.Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol. Meth. 65 : 55-63.
9.Gulcin, I., Buyukokuroglu, M. E., Oktay, M. and Kufrevioglu. O. I. 2003. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. Pallsiana (Lamb.) Holmboe. J. Ethnopharmacol. 86 : 51-8
10.Yena, G. C., Duhb, P. D. and Tsaia, L. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem. 79 : 307-313.
11.Ryu J. H., Ahn H, Kim, J. Y. and Kim, Y. K. 2003. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophage. Phytother. Res. 17 : 485-489.
12.Cho, Y. J. and Ahn, B. J. 2008. Anti-inflammatory effect of extracts from Cheongmoknosang (Morus alba L.) in lipopolysaccharide stimulated Raw cells. J. Kor. Soc. Appl. Biol. Chem. 51 : 44-48.
13.Winter, C. A., Risley, E. A. and Nuss, G. W. 1962. Carrageenan induced edema in the hind paw of rat as an assay for anti-inflammatory activit. Proc. Soc. Exp. Biol. Med. 111 : 544-547.
14.Leong, L. P. and Shui, G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76 : 69-75.
15.Choi, Y. M., Chang, W. B., Choi, S. Y., Choi, J. S., Noh, J. G., Song, I. K., Min, K. B. and Lee, J. S. 2008. Biological activities of Lyophyllum ulmarium extracts. J. Agri. Life Sci. 42 : 35-41.
16.Lee Y. L., Huang. G. W., Liang, Z. C. and Mau J. L. 2007a. Antioxidant properties of three extracts from Pleurotus citrinopileatus. LWT-Food Sci. Technol. 40 : 823-833.
17.Tsai, S. Y., Huang, S. J, and Mau, J. L. 2006. Antioxidant properties of hot water extracts from Agrocybe cylindracea. Food Chem. 98 : 670-677.
18.Mau, J. L., Chang, C. N., Huang, S. J. and Chen, C. C. 2004. Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta, and Termitomyces albuminosus mycelia. Food Chem. 87 : 111-118.
19.Lee, Y. L., Yen, M. and Mau, J. L. 2007b. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem. 104 : 1-9.
20.Kang, H. W. 2012. Antioxidant and anti-inflammatory effects of extracts from Flammulina velutipes (Curtis) Singer. J. Kor. Soc. Food Nutr. 41 : 1072-1078.
21.Lim, J. H., Kim, S. H., Park, N. H., Moon, C. G., Kang, S. S., Kim, S. H., Shin, D. H. and Kim, J. C. 2010. Acute and chronic antiinflammatory effects of Phellinus linteus water extract in rats J. Biomed. Res. 11 : 27-35.