갈색 느티만가닥버섯 메탄올 추출물의 항산화 및 tyrosinase 저해 효과
Antioxidant and tyrosinase inhibitory activity of Hypsizygus marmoreus (brown cultivar) methanol extracts
(Received December 11, 2013 / Revised December 23, 2013 / Accepted December 27, 2013)
Abstract
The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanolextracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) andextracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometricmethods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidantactivities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanolextract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinaseinhibitory activity of the methanol extract (200 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than thoseof positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus andstipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higherproliferation rate than those of 0.04% adenosine.
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Reference
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34.Zanabaatar, B., Kang, M. G., Seo, G. S., Lee, Y. W. and Lee, J. S. 2012. Analysis of nutritional characteristics and physiological functionality of Hypsizygus marmoreus (Brown cultivar). Kor. J. Mycol. 40 : 104-108.
35.Zhishen, J., Mengcheng, T. and Jianming, W. 1998. The determination flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 64 : 555-559.
2.Aburjai, T. and Natshen, F. M. 2003. Plants used in cosmetics. Phytother. Res. 17 : 987-1000.
3.Aitken, R. J., Bukingham, D. and Harkiss, D. 1993. Use of xanthine oxidase free radical generating system to investigate the cytotoxic effect of reactive oxygen species on human spermatozoa. J. Reprod. Fertil. 97 : 441-450.
4.Barros, L., Ferreira, M. J., Ferreira, I. C. F. R. and Baptista, P. 2007. Total phenols, ascorbic acid, β-carotene and lycopene in portuguese wild edible mushrooms and their antioxidant activities. Food Chem. 103 : 413-419.
5.Baublis, A. J., Lu, C., Clydesdale, F. M. and Decker, E. A. 2000. Potential of wheat-based breakfast grains as a source of dietary antioxidants. J. Am. Coll. Nutr. 19 : 308S-311S.
6.Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature. 181 : 1199-1200.
7.Branien, A. L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52 : 59-63.
8.Cha, J. Y. 2009. Functional components and biological activities of marketing black garlic. MS Thesis. Gyengsang National University.
9.Chang, S. T., Buswell, J. A. and Chiu, S. W. 1993. Mushroom biology and mushroom product. The Chinese University Press. Hong Kong pp. 3-17.
10.Chang, T. and Miles, P. G. 1989. Edible mushrooms and their cultivation. CRC press. pp. 27-40.
11.Chen, H. Y., Lin, Y. C. and Yen, G. C. 2007. Antioxidant activity and free radical scavenging capacity of extracts from guava (Psidium guauva L.) leaves. Food Chem. 101 : 686-694.
12.Droge, W. 2001. Free radicals in the physiological control of cell function. Physiol. Rev. 82 : 47-951.
13.Ferreira, I., Barros, L. and Abreu, R. 2009. Antioxidants in wild mushrooms. Current med. Chem. 16 : 1543-1560.
14.Feskanich, D., Ziegler, R. G., Michaud, D. S., Giovannucci, E. L., Speizer, F. E., Willett, W. C. and Colditz, G. A. 2000. Prospective study of fruit and vegetable consumption and risk of lung cancer among men and women. J. Natl. Cancer Inst. 92 : 1812-1823.
15.Francoeur, A. M. and Assalian, A. 1996. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica. 3 : 19-25.
16.Guk, M. H., Kim, D. H., Lee, C., Jeong, E. S., Choi, E. J., Lee, J. S. and Lee, T. S. 2013. Antioxidant and skin whitening effects of inonotus obliquus methanol extract. J. Mushroom Sci. Prod. 11 : 99-106.
17.Halliewell, B. and Gutterridge, J. M. 1990. Roles of free radicals and catalytic metal ions in human disease. An overreview, Methods Enzymol. Fleischer, S. and packer, L. (eds.). Academic Press, New York, USA. 186 : 1-12.
18.Hearing, V. J. and Ekel, T. M. 1976. Mammalian tyrosinase. Biochem. J. 157 : 549-557.
19.Hirase, S., Nakai, S. and Akstus, T. 1976. Sturucture studies on the antitumor active polysaccharides from Coriolus versicolor (Basidiomycetes). I. Fractionation with barium hydroxide. Yakugaku Zasshi. 96 : 413-418.
20.Ikekawa, T. 1995. Bunashimeji, Hypsizygus marmoreus antitumor activity of extractsand polysaccharides. Food Rev. Int. 11 : 207-209.
21.Kim, D. J., Oh, S. K., Yoon, M. R., Chun, A. R., Hong, H. C., Lee, J. S. and Kim, Y. K. 2010. Antioxidant compounds and antioxidant activities of the 70% ethanol extracts from brown and milled rice by cultivar. J. Korean Soc. Food Sci. Nutr. 39 : 467-473.
22.Langton, A. K., Sherratt, M. J., Griffiths, C. E. and Watson, R. E. 2010. A new wrinkle on old skin: the role of elastic fibres in skin ageing. Int. J. Cosmet. Sci. 32 : 330339.
23.Lee, M. and Oh, S. I. 2007. S Antioxidative stress and antimutagenic effects of Lentinus edodes ethanol extracts. Korean J. Food & Nutr. 20:341-348.
24.Lee, J. S., Bolormaa, Z., Kim, M. K., Seo, G. S. and Lee, Y. W. 2011. Screening and physiological functionality of Hypsizygus marmoreus (White Cultivar) fruiting body. Korean J of Mycol. 39 : 185-188.
25.Matsuzawa, T., Sano, M., Tomita, I., Saitoh, H., Ohkawa, M. and Ikekawa, T. 1998. Studies on antioxidants of Hypsizygus marmoreus. II. Effects of Hypsizygus marmoreus for antioxidants activites of tumor-bearing mice. Yakugaku Zasshi. 118 : 476-481.
26.Montonen, J., Knekt, P. R., Jarvinen, R., Aromaa, A. and Reunanen, A. 2003. Whole-grain and fiber intake the incidence of type 2 diabetes. Am. J. Clin. Nutr. 77 : 622-629.
27.Nita, A. and Young, A. R. 2005. Melanogenesis: A photoprotective response to DNA damage? Mutation Research. 571 : 121-132.
28.Ohashi, H. 2010. Trends of mushrrom production and marketing. InL "Annual report of mushroom 2010" (ed. by Ohashi, H.). p. 18. Plant's world Co. Ltd. Tokyo. Japan.
29.Singleton, V. L. 1981. Naturally occurring food toxicants: phenolic substances of plant origin common in foods. Adv. Food Res. 27 : 149-242.
30.Tomita, K., Oda, N., Ohbayashi, M., Kamei, H., Miyaki, T. and Oki, T. 1990. A new screening method for melanin biological synthesis inhibitor using Streptomyces bikiniensis. J. Antibiotics. 43 : 1601-1605.
31.Verckei, A., Toncsev, H., Feher, J. and Hajdu, E. 1992. Relationship between the extent of coronary artery disease and indicators of free radical activity. Clin. Cardiol. 15 : 706-707.
32.Yang, C. S., Landau, J. M., Hung, M. T. and Newmak, H. L. 2001. Inhibition of carcnogeneses by dietary polyphenolic compounds. Annu. Rev. Nutr. 21 :3 81-406.
33.Yoon, K. N., Lee, K. R., Shin, P. G., Cheong, J. C., Yoo, Y. B. and Lee, T. S. 2011. Antioxidant and antityrosianse activities of various extracts from the fruiting bodies of Lentinus lepideus. Molecules. 16 : 2334-2347.
34.Zanabaatar, B., Kang, M. G., Seo, G. S., Lee, Y. W. and Lee, J. S. 2012. Analysis of nutritional characteristics and physiological functionality of Hypsizygus marmoreus (Brown cultivar). Kor. J. Mycol. 40 : 104-108.
35.Zhishen, J., Mengcheng, T. and Jianming, W. 1998. The determination flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 64 : 555-559.