“Hwaseong 5ho” was developed by the method of Di-mon mating between monokaryotic strains derivedfrom “Hwaseong 1ho” and dikaryotic strain “PSC109”. The color of pileus was dark grayish brown, the shape of pileuswas convex or infundibuliform. The length of stipe was longer and the thickness of stipe was some thinner than Suhan1ho. Material properties of stipe of “Hwaseong 5ho” was higher in strength, hardness, chewingness and brittleness thanSuhan 1ho, but similar in elasticity and cohesion. RAPD using URP-primer showed not the same between two strains.Days of primordia formation period were 22-27 days after spawning, that was a little later than Suhan 1ho. In the trialusing culture box containing composted cotton waste, yield index of ‘Hwaseong 5ho’ was 16.6% higher than Suhan 1ho.The farm field trial were showed stable productivity in each different growing conditions.