This study was carried out to find out suitable input method for air exchange about bottle cultivation ofoyster mushroom. There was no difference of average temperature in cultivation room, but T1(direct introduction of outsideair) was higher than T2(heat exchanger) and T3(air buffer) in the standard deviation. The ratio of cooling operating wasthe highest in August and in the descending order, T1 54%, T3 43%, T2 33%. At ratio of energy reduction, T2 andT3 were higher than T1. The operating ratio of heater was highest in January and in the descending order, T1 53%,T3 37%, T2 30%. At ratio of energy reduction(%), T2 and T3 were higher than T1 similar to result of cooling operating.Therefore there were largest in August about Cooling Degree-Hour and in January about Heating Degree-Hour. And fruitbodyyields quality was excellent in T2 and T3 than T1. The suitable effective type of air exchange in oyster mushroomcultivation are heat exchange and air buffer system.