국내 표고버섯 주요 버섯파리의 분자생물학적 종 동정 및 발생양상
Molecular identification of fungus gnats from shiitake mushroom in Korea
(Received November 19, 2013 / Revised December 23, 2013 / Accepted December 30, 2013)
Abstract
Fungus gnats are usually found in mushroom farm and have recently become important pest because theycan cause severe damage and reduce the production on shiitake mushroom. Usually shiitake mushrooms are cultivatedon both oak bed logs and in the artificial sawdust beds in greenhouses. Using yellow sticky trap, the dipteran speciesin shiitake mushroom farm were collected from May to September in Kyonggi-do and Chungcheong-do in 2013. To identifythe main species of fungus gnat on the shiitake farm in Korea, the collected samples were determined the sequenceof cytochrome c oxidase subunit I (COI) by DNA barcoding. The phylogeny based on maximum likelihood analyses fromCOI sequence showed that Bradysia difformis and B. alpicola were main species of shiitake bed log and Scatopsidaesp. and B. difformis were dominant species of sawdust beds.
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Reference
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2.Armstrong, K.F., Ball, S.L., 2005. DNA barcodes for biosecurity: invasive species identification. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 360 : 1813-1823.
3.Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology. 3 : 294-299.
4.Jeanmougin, F., Thompson, J.D., Gibson, T.J., Plewniak, F., Higgins, D.G., 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 25 : 4876-4882.
5.Kim, K.C., Hwang, C.Y., 1996. An investigation of insect pest on the Mushroom (Lentinus edode, Pleurotus ostreatus) on south region of Korea. Kor. J. Appl. Entomol. 35 : 45-51.
6.Menzel, F., Smith, J.E., Colauto, N.B., 2003. Bradysia difformis Frey and Bradysia ocellaris (Comstock): two additional neotropical species of black fungus gnats (Diptera: Scairidae) of economic importance: a redescription and review. Ann. Entomol. 96 : 448-457.
7.Rivera, J., Currie, D.C., 2009. Identification of Nearctic black flies using DNA barcodes (Diptera: Simuliidae). Mol. Ecol. Resour. 9 : 224-236.
8.Shin, S., Jung, S., Lee, H., Lee, S., 2013. Molecular identification of dipteran pests (Diptera: Sciaroidea) from shiitake mushroom. Mol. Ecol. Resour. 13 : 200-209.
9.Shin, S.G., Lee, H.S., Lee, S.H., 2012. Dark winged fungus gnats (Diptera: Sciaridae) collected from shiitake mushroom in Korea. Journal of Asia-Pacific Entomology. 15 : 174-181.
10.Smith, M.A., Woodley, N.E., Janzen, D.H., Hallwachs, W., Hebert, P.D., 2006. DNA barcodes reveal cryptic hostspecificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proc. Natl. Acad. Sci. 103 : 3657-3662.
11.Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Bio.l Evol. 24 : 1596-1599.
12.Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28 : 2731-2739.