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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.11 No.4 pp.194-200
DOI :

The culture conditions for mycelial growth and sclerotial formation of Polyporus umbellatus

Tae Soo Lee2, Min Woong Lee1, Kwang Chun Chang1, Do Bin Shin2, Kyung Rim Lee2, Kyung Hoan Im2, Ga-Heon Jin4, Pyung Gyun Shin5, Yong Mei Xing3, Juan Chen3, Shun Xing Guo3
2Division of Life Sciences, Incheon National University, Incheon, 406-840, Korea
1Department of Life Science, Dongguk University, Seoul 100-715, Korea
3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, People's Republic of China.
4Department of Ophthalmic Optics, Shinheung University 95 Hoam-ro, Uijeongbu, Gyeongggi 480-701, Korea
5Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Korea
(Received December 7, 2013 / Revised December 27, 2013 / Accepted December 30, 2013)

Abstract

Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceaeof Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicinesin China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus.Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillariamellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture betweenP. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were 20℃ and pH 4, whileoptimal conditions for mycelial growth of A. mellea were 25oC and pH 6. The carbon sources for optimal mycelial growthof P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were alsofructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract,while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. melleawere dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose andmaltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeastextract for 60 days at 20℃ under dark condition.

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