Mycelial viability and cultivation characteristics of strains of Hypsizygus marmoreus after long-term storage wereinvestigated. The experimental conditions were the storage at 4oC using a slant culture technique with or without mineral oil, andin liquid nitrogen tank in the presence of 10% glycerol or 10% glycerol with 5% trehalose. The myceila of four strains of H.marmoreus were thawed at 9, 21, 33, and 45 months after beginning of the storage, and then the growth of the mycelia wasmeasured on a PDA plate with serial transfers to new plate for the recovery. The mycelial growth data after 45 months showedthat the mycelia were mostly viable but not fully active particularly when they were stored in liquid nitrogen with 10% glycerol.The growth activity could be fully recovered after second transfer to new PDA plate. Cultivation of mushroom fruiting body usingthe recovered mycelia also demonstrated that the storage methods employed in this work were applicable for the long-termstorage of H. marmoreus.