This study was carried out to develop the new mushroom cultivation medium for Pleurotus ostreatus using by EFB.Two strains of Pleurotus ostreatus were used for this study and the difference in productivity was observed in each strain. Therewas not confirmed a mycelial growth inhibition by addition of Empty Fruit Bunch which is palm tree waste. In the productivitytest of the mixed medium, In Suhan 1ho, yield of T2 treatment was highest as 90.6 g per bottle, It was 14.9% higher thancontrol. In Kimje variety which is Chunchu strain, Yield of T2 treatment was highest as 139.8 g per bottle. It was 25.2% higherthan control. In Kimje variety, as amount of 6mm diameter PEFB pellet is increased in medium, yield of mushroom wasdecreased. When 8mm diameter EFB was used for 532 medium, yield of both 50% EFB(T3) and 100% EFB (T5) treatment wasbetter than other treatments. Therefore, it is suggested that 8 mm diameter EFB pellet is good material for mushroom cultivationmedium and T5 treatment was showed a potential possibility as an alternative medium.