This study was initiated to investigate antioxidant, anti-acetylcholinesterase, and xanthine oxidase inhibitory activitiesand properties of fruiting bodies, mycelia, and fermentation culture filtrates from Phellinus igniarius. The contents of total phenolsand flavonoid of fruit bodies, mycelia, and culture filtrate were 15.35-1.36 mg/g, 10.35-7.85 mg/g, and 8.25-5.36 mg/g. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging abilities of the extracts from the fruiting bodies, mycelia, and culture filtrateswere 90.25-95.60%, 78.82-85.24%, and 76.32-82.50% at 50-400 μg/mL, respectively. The chelating ability of fruiting bodyextract on ferrous ions was higher than those of mycelia and culture filtrates tested. The anti-acetylcholinesterase inhibitoryactivity of the fruiting body extract at 400 μg/mg exhibited 91.10% on AChE, which is lower than that of positive control,galanthamine (94.82%). The xanthine oxidase inhibitory activities of the fruiting bodies, mycelia, and culture extract were85.47%, 78.13%, and 72.49% at 400 μg/mL, respectively. Overall, the fruiting body extract has better anti-acetylcholinesterase,antioxidant and xanthine oxidase inhibitory activities than those from mycelia and culture filtrate.