Studies were made to optimize the media composition in bag culture and conducted to determine the possibility ofartificial cultivation of Auricularia auricula. Sawdust spawn of media composition for optimal growth were found to be oaksawdust80%combination of 20% popla-sawdust were the best of the optimal combination. And optimal substrate combinationwere found to be cotton-seed meal combination of 10% wheat bran 5% mixed were the best of combination. The duration ofspawn run period and primordial formation period on bag(1.2 kg) were 50 days and 7 days, respectively. The weight of fruiting body and the yield(100ea, 1.2 kg) of fresh fruit-body were 24 g and 45,000 g, respectively.