Phylogenic relationships and morphological characteristics were classified and investigated among the 233 collectedstrains of Agaricus spp. The 38 strains were differently identified as different characteristic group using analysis of ITS regions inrDNA. A. bitorquis was showed close relationship in groupA whereas A. campestris was in groupC as different characteristic groupamong with A. bisporus. There was no phylogentic difference with strains collected by country and different pileus colored Agaricusbisporus. Also the strains were cultivated twice to investigate morphological characteristics of fruiting bodies. The characteristics andyield of collected strains were compared with molecular varieties and seasons by the cultivation. In this result, A. campestirsshowed good yield and quality in terms of hardness off-white mushroom was more harder than other number of A. bisporus. Alsoearliness and color of pileus was influenced by external environment all conditions