Pleurotus pulmonarius usually grows on the dead wood of deciduous trees from mid summer to early fall.Cultivation of this mushroom is recently increasing due to its good flavour and fragrance. This study was conducted to find outthe favorable substrate formulation in the bottle and bag cultivation of P. pulmonarius. The V formulation (10 : 50 : 20 : 20mixing ratio of poplar sawdust, cottonseed hull, cottonseed meal and beet pulp) showed the fastest mycelial growth in the testtube column medium and highest yields in both bottle cultivation and bag cultivation. Those chemical conditions were pH5.52, 29.07% organic matter contents, 1.12% total nitrogen, 25.95 OM/N ratio, 0.16% CaO, 0.51% K2O, 0.17 MgO and 0.26P2O5.