This study was to comparative analysis that different methods of extraction about total sugar content which themajor functional components of edible and medicinal mushrooms. Through the phenol-sulfuric acid method was measuredthat Hot water extraction, acid extraction, and alkaline extraction. Drawing the standard curve to obtain the concentrationof the glucose solution and the sample by applying the measured absorbance on the standard curve. Total sugar contentswas measured after extraction with different solvents.(Hot water, 70% EtOH, 80% MeOH). The level of total sugar inCordyceps militaris is highest in the hot water extraction with 658.4(μg/g) and Coriolus versicolor in 649.7(μg/g) and679.0(μg/g) was found as the highest in the acid extraction and the alkaline extraction. C. militaris and ASI 7025 showed thehighest level in the hot water extraction. Finally, C. versicolor, Sparassis crispa showed that higher than the other mushrooms atthe 1hr, 2hr microwave extraction. It was varied total sugar content according to the different extraction solvent and theextraction method.