For evaluating the anti-allergy activity of mushrooms, forty two mushroom extracts were studied their inhibitoryactivities on the IgE-mediated degranulation in rat basophilic leukemia cell line (RBL-2H3 cell). Effects of mushroom extracts onthe release of interleukin-4 (IL-4) and beta-hexosaminidase, and the cell viability of the IgE-sensitized were measured. From theanalysis, five mushroom extracts such as the water extract from Flammulina velutipes (Curt.:Fr.) Sing showed the suppressiveactivities on IL-4 release as 20%. Eight extracts including the water extract of Ganoserma lucidum showed the suppressiveactivities on β-hexosaminidase release as 20%. Almost all of the extracts stimulated the proliferation of RBL-2H3 cell. The waterextracts of Flammulina velutipes and Phellinus linteus were examined against the inhibitory activity in the production of IL-4 andβ-hexosaminidase. Additionally, the extracts from Ganoserma lucidum, Isaria japonica, Phellinus linteus and Pleurotus ostreatusinhibited dose-dependently on β-hexosaminidase production. In conclusion, the result suggests that the mushrooms with thepotent inhibitory efficacies on the degranulation of the mast cell would be candidate resources for the anti-allergy resources, andthus need to study for their utilization.