This study was focused on finding a relationship between fruit-body growth and relative humidity in Neolentinuslepideus cultivation. The higher the relative humidity, the moisture content of pileus and stipe were also tends to be increased.The day of formation of primordia and development of fruit body was shortest at the relative humidity 95%. The yield andcommercial yield were higher in the relative humidity 95% than in the other treatment. Individual weight of fruit body was thehighest at the relative humidity 75% and 85%. Crack indices of pileus was the strongest at the relative humidity 65%