Genetic relationship analysis by PCR reactions with 25 primers could produce relevant classifications of the 21 P. pulmonarius (Fr) Quel. isolates collected from 2005. They were classified by two groups with similarity coefficient 0.37. All of the P. pulmonarius cultivars belonged to group 2. New cultivar ‘Hwasan’ presented high similarity coefficient to parental strains, and that of ‘Hosan’ and ‘Kangsan’ cultivars was very high owing to shared crossing parents, and that of two strains from China was high, as well.