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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.13 No.2 pp.97-102
DOI : https://doi.org/10.14480/JM.2015.13.2.97

Optimum cultivation conditions for mass production of antagonistic bacterium Pseudomonas azotoformans HC5 effective in antagonistic of brown blotch disease caused by Pseudomonas tolaasii

Chan-Jung Lee*, Ji-Won Moon, Young-Mi Yoo, Ju-Yeon Han, Jong-Chun Cheong, Won-Sik Kong
Mushroom Research Division, NIHHS, RDA, Eumseong 369-873, Korea
**Corresponding author E-mail : lchanj@korea.kr Tel : +82-43-871-5711, Fax : +82-43-871-5702
June 15, 2015 June 23, 2015 June 29, 2015.

Abstract

This study was conducted to investigate optimum conditions for mass production of antagonistic microbes
Pseudomonas azotoformans HC5. P. azotoformans HC5 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This markedly showed the antagonistic activity against P. tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the P. azotoformans HC5, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 6.0 and 15oC, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium was determined as follows: 0.6% adonitol, 1.5% yeast extract, 0.8% NH4H2PO4, 5mM MgSO4, and 0.2% asparagine.

버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 대한 길항세균 Pseudomonas azotoformans HC5의 대량배양을 위한 최적 배양조건

이찬중*,문지원,유영미,한주연,정종천,공원식
농촌진흥청 국립원예특작과학원 버섯과

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