‘Daejang 3ho’ is a new cultivar of oyster mushroom for high productivity than that of Chunchu 2ho. This cultivarwas bred by Di-mon mating between monokaryotic strains isolated from ‘Daejang 1ho' and dikaryotic strain ‘Jangan 5ho’. Thecolor of pileus and the shape of pileus of new cultivar were dark gray and deeply funnel shape, respectively. The length andthickness of stipe were longer and thicker than those of Chunchu 2ho. It took 4~5 days to formation of primordia, that was asimilar to Chunchu 2ho. The optical temperature of fruit body was 14-17oC. Yield of ‘Daejang 3ho’ was 13.9% higher than thatof Chunchu 2ho. Analysis of RAPD by URP-primer of #03, #08, #10 and #11 showed that different band pattern those ofother strains. There were no significant differences between the texture properties of ‘Daejang 3ho’ and Chunchu 2ho.