Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates

Kyong-Jin Min^1,2, Won-Sik Kong³, Hee-Wan Kang^1,2,4,*

¹Graduate School of Future Convergence Technology, Hankyong National University, Ansung 456-749, Korea
²JK BioTech. Co. Ltd., Ansung 456-749, Korea
³Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea
⁴Institute of Genetic Engineering, Hankyong National University , Ansung 456-749, Korea

^*Corresponding author, E-mail : kanghw2@hknu.ac.kr, Tel:+82-31-670-5420, Fax:+82-31-670-5469

Abstract

Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strainsincluding 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCRpolymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted forUPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea wereinvolved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains weregenetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCRpolymorphism could potentially be used to identify homokaryon isolates.

Inter simple sequence repeat (ISSR)-PCR에 의한 양송이버섯(Agaricus bisporus) 계통과 단핵균주의 다형성 분석

민경진^1,2·공원식³·강희완^1,2,4,*

¹한경대학교 미래융합기술대학원
²㈜ 제이케이바이오테크
³농촌진흥청 국립특작과학원 버섯과
⁴한경대학교 유전공학연구소

Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates

Abstract

Inter simple sequence repeat (ISSR)-PCR에 의한 양송이버섯(Agaricus bisporus) 계통과 단핵균주의 다형성 분석

초록

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