This study was conducted to obtain a growth correlation of basal information from the development of disease resistantFlammulina velutipes cultivars through back-crossing between the strains of wild-type brown monokaryon 4019-20 and thederivative of commercial quality white monokaryons 3. The two strains were selected to back-cross for further enhancing their latentattributes and growth characteristics. The parents of 4019-20×M3 back-crossed to reproduce F1, M3-Sn. Using F1, M3-Sn procuredand isolated into 94 monokaryon strains. Further examination of growth characteristics carried out by back-crossing between M3and BC1F1 from M3-n dikaryon. Monokaryon exhibited an irregular growth pattern and demonstrated to be sluggish development inthe sawdust medium. However BC1F1(M3-n) dikaryon strains confirmed mostly regular growth pattern and demonstrated ordinarygrowth in the sawdust medium. The fruitbody of BC1F1 confirmed as light-brown colour to be the dominant gene. The colourdistributions of fruitbody, BC1F1, resulted as follows; 7% of dark brown, 25% of brown , 27% of light brown, 16% of ivory and 25%of white. The ratio of the other color to white showed 3 to 1 which suggested two major genes were related to fruitbody color.