This study was carried out to investigate the optimum rate of substrate composition in bottle cultivation of Grifola frondosa and had three rates of substrate composition of 67:11:22(T1), 68:15:17(T2) and 74:14:12(T3) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse. The rate of primordia formation of T3 was 65.8% which was lowest among all treatments. Contraction rate of disease of T1 was 9.8% which was highest among all treatments. Harvesting rate of T2 was 70.5% which was highest among all treatments. Fruit body weights per bottle of T1 and T2 were 85.5 g, 83.3 g respectively and there was not significant difference between those. Yield per 10,000 bottles of T2 was 587 kg and was 7%, 28% higher than those of T1 and T3, respectively. As a result, the rate of substrate composition of 68:15:17(T2) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse was appeared as optimum rate of substrate composition in bottle cultivation of Grifola frondosa..

본 연구는 잎새버섯 병 재배시 적합한 배지조성비율을 구명하고자 수행되었다. 배지조성은 참나무톱밥: 옥수수피: 건비지를 건물중량 비 율로 T1처리(67:11:22), T2처리(68:15:17), T3처리(74:14:12)의 3처리를 두고 시험한 결과, 발이율은 T1처리에서 72.6%, T2처리에서 72.1%로 두 처리 간 유의적 차이가 없었으나 T3처리에서는 65.8%로서 다른 두 처리에 비하여 낮았다. 이병률은 T2처리에서 4.1%, T3처리에서 3.7%인데 비하 여 T1처리에서는 9.8%로 가장 높았다. 수확률은 T1처리 에서 64.1%, T3처리에서 63.6%인데 비하여 T2처리에서 는 70.5%로 가장 높았다. 병당 자실체 중량은 T1처리에 서 85.5 g, T2처리에서 83.3 g으로 두 처리 간 유의적 차 이가 없었으나 T3처리에서는 72.4 g으로 다른 두 처리에 비하여 낮았다. 입병 수 10,000병 기준으로 재배 시 수량은 T2처리에서 587 kg으로 T1처리 및 T3처리에 비하여 각각 7% 및 28%가 높았다. 따라서 잎새버섯 병재배 시 적합한 배지조 성비율은 건조중량비율로 참나무톱밥: 옥수수피: 건비지 가 68:15:17인 것으로 나타났다.