This study was performed to determine most appropriate mixed ratio of brewers dried grain to wheat bran as a nutrient supplement in the plastic vinyl bag cultivation of Grifola frondosa. In the examination of an appropriate nutrient source, the IV substrate formulation (80 : 10 : 10, oak sawdust : wheat bran : brewers dried grain) resulted in 71.6 days to harvest and the highest yield of 142.6 g/bag. On the other hand, the investigation of the optimal mixing ratio of beer waste to wheat bran showed that the III substrate formulation (80 : 5 : 15, oak sawdust : wheat bran : brewers dried grain) resulted in the least days to harvest (61.8 days), with the highest yield of 140.8 g/bag.