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ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.15 No.3 pp.111-117

Varietal characteristics of new Cordyceps militaris
‘Dowonhongcho 2ho’ improved by mating type molecular markers

Byung-joo Lee1*, Mi-Ae Lee1, Yong-Gyun Kim1, Sun-Gye Lee1, Young-sang Choi2, Byung-eui Lee3
1Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services, Yesan 340-861, Korea
2Yedang Mushroom Institute, Yesan 340-861, Korea
3Department of Chemistry, College of Natural Science, Soonchunghyang University, Asan 336-745, Korea
*Corresponding author
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The mushroom species Cordyceps militaris has been studied and cultivated as a medicinal mushroom due to its multiple valuable biological and pharmaceutical activities. For breeding new strains of C. militaris, multiplex PCR assays were performed using primers specific for its mating type genes, MAT1-1 and MAT1-2. Mating types and mating status were confirmed, as evidenced by DNA bands at 233-bp and 191-bp for MAT1-1 and MAT1-2 respectively. The novel 'Dowonhongcho 2ho’ was developed through mating; they were found to possess high-quality fruiting bodies when grown in artificial media. The stromata of the new strain were club-shaped, with a bright orange-red color, and measured 7.1 cm in length. They had an average cordycepin content of 0.33%. Compared to 'Dowonhongcho,' the new strain had a 7% higher yield, as well as firm fruiting bodies. The optimum temperature for mycelial growth was 20~25°C, and the optimum temperature for stroma development was 18~22 °C. The fruiting bodies developed after 49.1 days from inoculation. The use of mating type molecular markers improved the breeding efficiency of the new strain 'Dowonhongcho 2ho.’ Thus, they may be valuable for artificial cultivation and industrial-scale production of C. militaris with excellent characteristics.

교배형 분자마커를 이용한 신품종 밀리타리스 동충하초 ‘도원홍초 2호’의 품종 특성

이병주1*, 이미애1, 김용균1, 이순계1, 최영상2, 이병의3
1충청남도농업기술원 작물연구과
3순천향대학교 화학과