Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.15 No.3 pp.139-144

Analysis of Inter-simple sequence repeat (ISSR) markers in
cultivars and collected strains of button mushroom(Agaricus bisporus)

Youn-keol Nam1,2, Won-Sik Kong1, Kab-Yeul Jang1, Pyung-Gyun Shin1, MinJi Oh1, Ji-Hoon Im1, Chang-Duck Koo2, Youn-Lee Oh1*
1Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea
2Department of Forestry, Chungbuk National University, Cheongju, 361-763
*Corresponding author
E-mail :
Tel : +82-43-871-5707


A. bisporus is the fifth most cultivated mushroom in Korea, and approximately 10,757 tons were cultivated in 2015. The genetic diversity of collected strains in Korea and commercial cultivars was analyzed using inter-simple sequence repeat (ISSR) markers. ISSR markers known to be comparable among A. bisporus spp. were selected from various markers. Totally, 16 markers, namely the ISSR markers 807, 808, 810, 811, 834, 835, 836, 841, 842, P3, P8, P17, P22, P30, P38, and P39, were evaluated to discriminate between ASI 1110, 1114, 1115, 1238, 1246, 1365, 1366, and 1369 for selecting suitable markers in 16 markers. The ISSR markers P31, P38 and P39 exhibited various fingerprints that could help classify the strains in species. Using the three markers, genetic relationships among 39 strains, including commercial cultivars, such as SaeA and SaeYeon, were analyzed using the UPGMA method. The results of the analysis of the genetic relationships between commercial cultivars and collected strains in Korea confirmed that the commercial cultivars were different from the collected strains in Korea. These results suggested that the ISSR markers P31, P38, and P30 could be used for selecting the commercial cultivars of A. bisporus.

양송이 품종과 수집 균주간의 Inter-simple sequence repeat(ISSR) 마커 분석

남윤걸1,2, 공원식1, 장갑열1, 신평균1, 오민지1, 임지훈1, 구창덕2, 오연이1*
1농촌진흥청 국립원예특작과학원 버섯과
2충북대학교 산림학과