This study was carried out to develop a method for cultivation of Lentinula edodes that would reduce both labor and waste. Cultural characteristics were studied, and mushroom yields were estimated to identify the most suitable method to culture L. edodes, using bottles and boxes. Among these, box cultivation with 7 kg of substrate had 40 days of browning period and the maximum yield of 945 g fruiting body; its biological efficiency was 32.7%. In contrast, box cultivation after pre-culture, and the bottle cultivation were found to be unsuitable for the production of L. edodes, due to long periods of browning, and low yields with poor quality, respectively. Further studies on box cultivation of L. edodes are necessary for commercial application of this method.