Mushrooms growing spontaneously in the Juwangsan National Park were surveyed from April to October 2017 for supporting management of higher fungi and creating a bio-diversity database of the park. In total, two divisions, eight classes, 21 orders, 85 families, 225 genera, and 503 species were detected. Among them, 247 species belonged to order Agaricales, which occupied 48.8% of the population, followed by members of orders Polyporales and Russulales. The mushrooms were abundant in August, and an altitude of 300-400 m above sea level was optimal for their growth, such as those found at Sanguijigu, Deajeonsa, and Geodaeri semgoal areas. Furthermore, Macrolepiota procera and Sarcodon aspratus, which belong to criteria of climate change, were detected in this survey.