A new blackish-brown variety of Flammulina velutipes, ‘Yeoreumhyang2ho,’ which was bred by crossing two monokaryons isolated from the ‘Heukhyang’ and ‘Garlmoe’ varieties, showed adaptability to high temperature. During bottle cultivation, the temperature was maintained at 16°C and the variety displayed good productivity compared to the control (‘Heukhyang’), without necessitating low temperature (4°C) treatment. The period necessary for mycelial and fruit body growth, and primordia formation was 24 days, 8 days, and 8 days, respectively. The total cultivation period was 40 days, which was 3 days shorter than that of the control. Particularly, primordia formation took 2 days less than the control and showed initial uniformity. The yield of ‘Yeoreumhyang2ho’ was 154 g per 850 ml bottle, which was 8% higher than the control yield. The β- glucan content was 67.9 g per 100 g dry weight, which is approximately 1.1–2.2 times the amount of β-glucan in control, the golden and white mushrooms that are available in the market.