This study investigated the proximate composition and total amino acid contents in Tricholoma matsutake and Sarcodon aspratus to compare the food quality according to production area. The crude ash contents in Tricholoma matsutake from Yanji (China), Yangyang (Korea), Tibet (China), and Yunnan (China) were 6.95%, 6.40%, 5.52%, and 5.40%, respectively. Yangyang showed the lowest crude fat content (1.19%), whereas Tibet exhibited the lowest crude protein content (16.83%), showing a difference according to the production area. The total amino acid content of Tricholoma matsutake in Yanji, Yunnan, Tibet, and Yangyang was 11,490.14±892.07 mg%, 8,000.03±207.25 mg%, 6,815.48 mg%, and 6,074.74±814.86 mg%, respectively. The contents of proximate composition and total amino acids in Sarcodon aspratus showed no difference according to production area. The results of proximate composition in Tricholoma matsutake suggest that the crude ash content can be used as a distinguishing indicator between Tricholoma matsutake from Yangyang and Tibet. Further studies are needed to analyze the mineral contents for the establishment of distinguishing mineral indicators between Tricholoma matsutake from Yangyang and Tibet.