This study investigated the characteristics of different cultivars of Lentinula edodes in sawdust culture at different cultivation days. Between cultivation days 30-120, the color of ‘Sanjo701ho’ displayed a reduction in L value (brightness) from 83.8±2.5 to 45.7±2.3, values a and b increased, but hardness decreased from 9.4±0.9 g/mm to 2.6±0.2 g/mm. Between cultivation days 30-120, ‘Nongjingo’ displayed a reduction in L value from 86.2±2.1 to 53.4±1.3. Values a and b increased with longer cultivation; however, hardness decreased from 4.8±0.7 g/mm on day 30 to 3.8±1.0 g/mm on day 120. ‘Sanjo701ho’ was first harvested at 46 days after a 30-, 89 days after a 60-, 8 days after a 90- (the shortest), and 9 days after a 120-day cultivation. The average fruit body weight was the highest on day 90 of cultivation, at 48.3 g, when the diameter and thickness of the mushroom cap also appeared highest. However, the colorimetric results showed that fruiting bodies produced in the culture medium for 120 cultivation days showed the most excellent commercial properties. ‘Nongjingo’ was first harvested at 22 days after a 30-, 18 days after a 60-, 8 days after a 90- (the shortest), and 9 days after a 120-day cultivation. Therefore, this study determined that a stable quantity of mushrooms with high commerciality can be produced with 120 cultivation days, considering the shiitake culture and the characteristics of the fruit body.