Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 1738-0294(Print)
ISSN : 2288-8853(Online)
Journal of Mushrooms Vol.22 No.4 pp.167-172
DOI : http://dx.doi.org/10.14480/JM.2024.22.4.167

Analysis of Tricholoma matsutake genetic diversity in Hongcheon, Republic of Korea, using SSR markers

Min-Jun Kim1, Aruna Jo1, Hee-Su Kim2, Yeongseon Jang1, Kang-Hyeon Ka1, Mi-Jeong Park1,*
1Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Republic of Korea
2Forestry Farm, Hongcheon 25128, Republic of Korea

Abstract

Tricholoma matsutake is a commercially valuable fungus that produces gourmet mushrooms. The artificial cultivation method that is currently used to produce fruiting bodies involves the transplantation of pine trees infected with T. matsutake from their natural habitats and the successful infection of young pine trees and mushroom production have been reported in Hongcheon. In this study, T. matsutake genetic diversity and relationships in this region were investigated. A genotype analysis was conducted on 25 fruiting bodies collected from an area approximately 2.56 km2 in size using 10 simple sequence repeat (SSR) markers. The analysis revealed 25 genotypes and 23 alleles with mean observed (HO) and expected (HE) heterozygosities of 0.312 and 0.293, respectively. The average polymorphism information content (PIC), which indicates marker polymorphism, was 0.257. A phylogenetic analysis showed no clear correlation between collection location and genetic distance; a spatial autocorrelation analysis indicated no significant genetic structure within 1600 m; and the Mantel test showed no significant correlation between genetic and geographic distances. These results were consistent with previous reports on Japanese populations. This study provides insights into the genetic diversity and structure of T. matsutake at a local scale and highlights the need for further studies across broader geographical ranges.

SSR 마커를 이용한 홍천 지역 송이의 유전다양성 분석

초록

Figure

Table