This study was analyzed by HPLC that the changing sugar contents according to the strains of Agaricus bisporus andcultivation temperature. All strains showed that high total amino acid content in 19oC. 103 strain have been identified as thelowest total amino acids and amino acids content compare to the other strains. The amino acid ‘cysteine’ content is the highest,followed by phenylalanine, glutamic acid, lysine, proline, histidine. This big difference is estimated because in the strains of A.bisporus and cultivated temperature.