The composition and content of amino acid and free amino acid were separately compared in cap and stipe offruitbody obtained from different color strains in winter mushroom and oyster mushroom strains. The result showed differentkinds and amount of amino acids according to the mushroom parts, strains and kinds. Tryptophane was not detected in anymushrooms. Alanine was also not detected in all oyster mushroom strains. The content of glutamic acid was the highest andthat of methionine, phenylalanine and cysteine were very low in all mushroom strains. When compared by parts, the ratio ofamono acids showed similar tendency in composition. Generally the content of amono acids showed higher in caps than instipes. The profile and content of free amino acid showed a lot of variation between cap and strip in the intra and intermushrooms.